Ontology highlight
ABSTRACT:
SUBMITTER: Cox DBT
PROVIDER: S-EPMC5793859 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Cox David B T DBT Gootenberg Jonathan S JS Abudayyeh Omar O OO Franklin Brian B Kellner Max J MJ Joung Julia J Zhang Feng F
Science (New York, N.Y.) 20171025 6366
Nucleic acid editing holds promise for treating genetic disease, particularly at the RNA level, where disease-relevant sequences can be rescued to yield functional protein products. Type VI CRISPR-Cas systems contain the programmable single-effector RNA-guided ribonuclease Cas13. We profiled type VI systems in order to engineer a Cas13 ortholog capable of robust knockdown and demonstrated RNA editing by using catalytically inactive Cas13 (dCas13) to direct adenosine-to-inosine deaminase activity ...[more]