Ontology highlight
ABSTRACT:
SUBMITTER: Bustin S
PROVIDER: S-EPMC5702850 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Biomolecular detection and quantification 20171122
Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative detection of amplification targets. Despite the framework provided by the MIQE guidelines and the accessibility of wide-ranging support from peer-review ...[more]