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In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device.

ABSTRACT: 3D tissue models are increasingly being implemented for drug and toxicology testing. However, the creation of tissue-engineered constructs for this purpose often relies on complex biofabrication techniques that are time consuming, expensive, and difficult to scale up. Here, we describe a strategy for realizing multiple tissue constructs in a parallel microfluidic platform using an approach that is simple and can be easily scaled for high-throughput formats. Liver cells mixed with a UV-crosslinkable hydrogel solution are introduced into parallel channels of a sealed microfluidic device and photopatterned to produce stable tissue constructs in situ. The remaining uncrosslinked material is washed away, leaving the structures in place. By using a hydrogel that specifically mimics the properties of the natural extracellular matrix, we closely emulate native tissue, resulting in constructs that remain stable and functional in the device during a 7-day culture time course under recirculating media flow. As proof of principle for toxicology analysis, we expose the constructs to ethyl alcohol (0-500 mM) and show that the cell viability and the secretion of urea and albumin decrease with increasing alcohol exposure, while markers for cell damage increase.

SUBMITTER: Skardal A

PROVIDER: S-EPMC5704969 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device.

Skardal Aleksander A Devarasetty Mahesh M Soker Shay S Hall Adam R AR

Biofabrication 20150910 3

3D tissue models are increasingly being implemented for drug and toxicology testing. However, the creation of tissue-engineered constructs for this purpose often relies on complex biofabrication techniques that are time consuming, expensive, and difficult to scale up. Here, we describe a strategy for realizing multiple tissue constructs in a parallel microfluidic platform using an approach that is simple and can be easily scaled for high-throughput formats. Liver cells mixed with a UV-crosslinka ...[more]

PMID: 26355538

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Dataset Information

In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device.

Publications

In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device.

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