Enzyme repurposing of a hydrolase as an emergent peroxidase upon metal binding.
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ABSTRACT:
SUBMITTER: Fujieda N
PROVIDER: S-EPMC5707476 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Enzyme repurposing of a hydrolase as an emergent peroxidase upon metal binding.
Fujieda Nobutaka N Schätti Jonas J Stuttfeld Edward E Ohkubo Kei K Maier Timm T Fukuzumi Shunichi S Ward Thomas R TR
Chemical science 20150507 7
As an alternative to Darwinian evolution relying on catalytic promiscuity, a protein may acquire auxiliary function upon metal binding, thus providing it with a novel catalytic machinery. Here we show that addition of cupric ions to a 6-phosphogluconolactonase <b>6-PGLac</b> bearing a putative metal binding site leads to the emergence of peroxidase activity (<i>k</i><sub>cat</sub> 7.8 × 10<sup>-2</sup> s<sup>-1</sup>, <i>K</i><sub>M</sub> 1.1 × 10<sup>-5</sup> M). Both X-ray crystallographic and ...[more]
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