Ontology highlight
ABSTRACT:
SUBMITTER: van der Vlugt-Bergmans CJ
PROVIDER: S-EPMC91782 | biostudies-literature | 2000 Jan
REPOSITORIES: biostudies-literature
van der Vlugt-Bergmans C J CJ Meeuwsen P J PJ Voragen A G AG van Ooyen A J AJ
Applied and environmental microbiology 20000101 1
We screened an Aspergillus tubingensis expression library constructed in the yeast Kluyveromyces lactis for xylogalacturonan-hydrolyzing activity in microwell plates by using a bicinchoninic acid assay. This assay detects reducing carbohydrate groups when they are released from a carbohydrate by enzymatic activity. Two K. lactis recombinants exhibiting xylogalacturonan-hydrolyzing activity were found among the 3,400 colonies tested. The cDNA insert of these recombinants encoded a 406-amino-acid ...[more]