Albumin adsorption onto surfaces of urine collection and analysis containers.

ABSTRACT:

Background

Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations.

Methods

We added (125)I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting.

Results

Adsorption of urine albumin (UA) at 5-6 mg/l was <0.9%; and at 90 mg/l was <0.4%. Adsorption was generally less at pH8 than pH5 but only 3 cases had p<0.05. Adsorption from 11 unaltered urine samples with albumin 5-333 mg/l was <0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100 ml and 2l collection containers, and to instrument sample cups and showed <1% change in concentration at 5 m

SUBMITTER: Robinson MK 

PROVIDER: S-EPMC5708857 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature