Ontology highlight
ABSTRACT:
SUBMITTER: Jacobs EZ
PROVIDER: S-EPMC5709416 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Scientific reports 20171130 1
The combination of genome-edited human embryonic stem cells (hESCs) and subsequent neural differentiation is a powerful tool to study neurodevelopmental disorders. Since the naïve state of pluripotency has favourable characteristics for efficient genome-editing, we optimized a workflow for the CRISPR/Cas9 system in these naïve stem cells. Editing efficiencies of respectively 1.3-8.4% and 3.8-19% were generated with the Cas9 nuclease and the D10A Cas9 nickase mutant. Next to this, wildtype and ge ...[more]