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Carbon fixation from mineral carbonates.

ABSTRACT: Photoautotrophs assimilate oxidized carbon obtained from one of two sources: dissolved or atmospheric. Despite its size, the pool of lithospheric carbonate is not known to be a direct source for autotrophy. Yet, the mechanism that euendolithic cyanobacteria use to excavate solid carbonates suggests that minerals could directly supply CO₂ for autotrophy. Here, we use stable isotopes and NanoSIMS to show that the cyanobacterium Mastigocoleus testarum derives most of its carbon from the mineral it excavates, growing preferentially as an endolith when lacking dissolved CO₂. Furthermore, natural endolithic communities from intertidal marine carbonate outcrops present carbon isotopic signatures consistent with mineral-sourced autotrophy. These data demonstrate a direct geomicrobial link between mineral carbonate pools and reduced organic carbon, which, given the geographical extent of carbonate outcrops, is likely of global relevance. The ancient fossil record of euendolithic cyanobacteria suggests that biological fixation of solid carbonate could have been relevant since the mid-Proterozoic.

SUBMITTER: Guida BS

PROVIDER: S-EPMC5715163 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Carbon fixation from mineral carbonates.

Guida Brandon S BS Bose Maitrayee M Garcia-Pichel Ferran F

Nature communications 20171018 1

Photoautotrophs assimilate oxidized carbon obtained from one of two sources: dissolved or atmospheric. Despite its size, the pool of lithospheric carbonate is not known to be a direct source for autotrophy. Yet, the mechanism that euendolithic cyanobacteria use to excavate solid carbonates suggests that minerals could directly supply CO<sub>2</sub> for autotrophy. Here, we use stable isotopes and NanoSIMS to show that the cyanobacterium Mastigocoleus testarum derives most of its carbon from the ...[more]

PMID: 29044115

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Project description:Autotrophic carbon fixation is a crucial process for sustaining life on Earth. To date, six pathways, the Calvin-Benson-Bassham cycle, the reductive tricarboxylic acid cycle, the 3-hydroxypropionate bi-cycle, the Wood-Ljungdahl pathway, the dicarboxylate/4-hydroxybutyrate cycle, and the 4-hydroxybutyrate cycle, have been described. Nano-organisms such as members of the Candidate Phyla Radiation (CPR) bacterial superphylum and the Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohalorchaeota (DPANN) archaeal superphylum could deeply impact carbon cycling and carbon fixation in ways that are still to be determined. CPR and DPANN are ubiquitous in the environment but understudied; their gene contents are not exhaustively described; and their metabolisms are not yet fully understood. Here, the completeness of each of the above pathways was quantified and tested for the presence of all key enzymes in nano-organisms from across the World Ocean. The novel marine ultrasmall prokaryotes were demonstrated to collectively harbor the genes required for carbon fixation, in particular the "energetically efficient" dicarboxylate/4-hydroxybutyrate pathway and the 4-hydroxybutyrate pathway. This contrasted with the known carbon metabolic pathways associated with CPR members in aquifers, where they are described as degraders (Castelle CJ, et al. 2015. Genomic expansion of domain archaea highlights roles for organisms from new phyla in anaerobic carbon cycling. Curr Biol. 25(6):690-701; Castelle CJ, et al. 2018. Biosynthetic capacity, metabolic variety and unusual biology in the CPR and DPANN radiations. Nat Rev Microbiol. 16(10):629-645; Anantharaman K, et al. 2016. Thousands of microbial genomes shed light on interconnected biogeochemical processes in an aquifer system. Nat Commun. 7:13219.). Our findings suggest that nano-organisms have a broader contribution to carbon fixation and cycling than currently assumed. Furthermore, CPR and DPANN superphyla are possibly not the only nanosized prokaryotes; therefore, the discovery of new autotrophic marine nano-organisms by future single cell genomics is anticipated.

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Carbon fixation from mineral carbonates.

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Carbon fixation from mineral carbonates.

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