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Palladium-Mediated Synthesis of a Near-Infrared Fluorescent K+ Sensor.

ABSTRACT: Potassium (K+) exits electrically excitable cells during normal and pathophysiological activity. Currently, K+-sensitive electrodes and electrical measurements are the primary tools to detect K+ fluxes. Here, we describe the synthesis of a near-IR, oxazine fluorescent K+ sensor (KNIR-1) with a dissociation constant suited for detecting changes in intracellular and extracellular K+ concentrations. KNIR-1 treatment of cells expressing voltage-gated K+ channels enabled the visualization of intracellular K+ depletion upon channel opening and restoration of cytoplasmic K+ after channel closing.

SUBMITTER: Bandara HMD

PROVIDER: S-EPMC5715468 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Palladium-Mediated Synthesis of a Near-Infrared Fluorescent K+ Sensor.

Bandara H M Dhammika HMD Hua Zhengmao Z Zhang Mei M Pauff Steven M SM Miller Stephen C SC Davie Elizabeth A Colby EAC Kobertz William R WR

The Journal of organic chemistry 20170714 15

Potassium (K+) exits electrically excitable cells during normal and pathophysiological activity. Currently, K+-sensitive electrodes and electrical measurements are the primary tools to detect K+ fluxes. Here, we describe the synthesis of a near-IR, oxazine fluorescent K+ sensor (KNIR-1) with a dissociation constant suited for detecting changes in intracellular and extracellular K+ concentrations. KNIR-1 treatment of cel ...[more]

PMID: 28664732

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