Project description:To clarify the effects of near-infrared radiation, we assessed DNA microarray after water-filtered near-infrared (1100-1800 nm together with a water-filter that excludes wavelengths 1400-1500 nm) irradiation.
Project description:To clarify the effects of near-infrared radiation, we assessed DNA microarray after water-filtered broad-spectrum near-infrared (1100-1800 nm together with a water-filter that excludes wavelengths 1400-1500 nm) irradiation.
Project description:To clarify the effects of near-infrared radiation, we assessed DNA microarray after water-filtered broad-spectrum near-infrared (1100-1800 nm together with a water-filter that excludes wavelengths 1400-1500 nm) irradiation. We performed 5 rounds of near-infrared irradiation (at 10 J âcm2) using 2 sets of transparent polycarbonate plates, one to block UV and the other to block both UV and near-infrared.
Project description:Near-infrared (NIR) fluorescent reporters open interesting perspectives for multiplexed imaging with higher contrast and depth using less toxic light. Here, we propose nirFAST, a small (14 kDa) chemogenetic NIR fluorescent reporter, displaying higher cellular brightness compared to top-performing NIR fluorescent proteins. nirFAST binds and stabilizes the fluorescent state of synthetic cell permeant fluorogenic chromophores (so-called fluorogens), otherwise dark when free. nirFAST displays tunable NIR, far-red or red emission through change of fluorogen. nirFAST allows imaging and spectral multiplexing in live cultured mammalian cells, chicken embryo tissues and zebrafish larvae. Its suitability for stimulated emission depletion nanoscopy enabled protein imaging with subdiffraction resolution in live cells. nirFAST enabled the design of a two-color cell cycle indicator for monitoring the different phases of the cell cycle. Finally, bisection of nirFAST allowed the design of a chemically induced dimerization technology with NIR fluorescence readout, enabling the control and visualization of protein proximity.
Project description:The purpose of this study is to determine if Near-Infrared fluorescence imaging is an effective approach to detect the colorectal tumoral tissues and peritoneal implants in colorectal cancer patients.
Project description:The sentinel lymph node (SLN) procedure is a standard staging technique in several types of cancer. One of the major problems of SLN mapping in colorectal cancer is the lack of an optimal dye and technique for identification of the nodes. In this study the investigators used the Near-Infrared (NIR) dye Indocyanin Green (ICG) to identify nodes with a newly developed NIR laparoscope. The investigators compared two different injection techniques; subserosal and submucosal injection.
Patients planned for a laparoscopic resection of a colorectal carcinoma without distant metastases were included. Dye was injected in the subserosa or submucosa of the bowel. Ten minutes after injection the investigators searched for fluorescent nodes with the NIR laparoscope. Fluorescent nodes were harvested and analyzed by the pathologist using H&E and additional immunohistochemistry.
Project description:Interventions: experimental group:application of near infrared-indocyanine green imaging system;Control group:Not use near infrared-indocyanine green imaging system
Primary outcome(s): Number of lymph nodes detected and positive rate;Perfusion of anastomosis;Anastomotic leakage;Navigation of vessels and structures
Study Design: Non randomized control