Ontology highlight
ABSTRACT:
SUBMITTER: Helms HC
PROVIDER: S-EPMC5718321 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Helms Hans Cc HC Aldana Blanca I BI Groth Simon S Jensen Morten M MM Waagepetersen Helle S HS Nielsen Carsten U CU Brodin Birger B
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 20170201 12
The aim was to characterize the clearance pathways for L-glutamate from the brain interstitial fluid across the blood-brain barrier using a primary in vitro bovine endothelial/rat astrocyte co-culture. Transporter profiling was performed using uptake studies of radiolabeled L-glutamate with co-application of transporter inhibitors and competing amino acids. Endothelial abluminal L-glutamate uptake was almost abolished by co-application of an EAAT-1 specific inhibitor, whereas luminal uptake was ...[more]