Ontology highlight
ABSTRACT:
SUBMITTER: Matson JP
PROVIDER: S-EPMC5720591 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Matson Jacob Peter JP Dumitru Raluca R Coryell Philip P Baxley Ryan M RM Chen Weili W Twaroski Kirk K Webber Beau R BR Tolar Jakub J Bielinsky Anja-Katrin AK Purvis Jeremy E JE Cook Jeanette Gowen JG
eLife 20171117
Complete and robust human genome duplication requires loading minichromosome maintenance (MCM) helicase complexes at many DNA replication origins, an essential process termed origin licensing. Licensing is restricted to G1 phase of the cell cycle, but G1 length varies widely among cell types. Using quantitative single-cell analyses, we found that pluripotent stem cells with naturally short G1 phases load MCM much faster than their isogenic differentiated counterparts with long G1 phases. During ...[more]