Ontology highlight
ABSTRACT:
SUBMITTER: Mukhopadhyay D
PROVIDER: S-EPMC5720925 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Mukhopadhyay Dwaipayan D Nadaud Philippe S PS Shannon Matthew D MD Jaroniec Christopher P CP
The journal of physical chemistry letters 20171120 23
We demonstrate rapid quantitative measurements of site-resolved paramagnetic relaxation enhancements (PREs), which are a source of valuable structural restraints corresponding to electron-nucleus distances in the ∼10-20 Å regime, in solid-state nuclear magnetic resonance (NMR) spectra of proteins containing covalent Cu<sup>2+</sup>-binding tags. Specifically, using protein GB1 K28C-EDTA-Cu<sup>2+</sup> mutant as a model, we show the determination of backbone amide <sup>15</sup>N longitudinal and ...[more]