Ontology highlight
ABSTRACT:
SUBMITTER: Moschen T
PROVIDER: S-EPMC4353840 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Moschen Thomas T Wunderlich Christoph Hermann CH Spitzer Romana R Levic Jasmin J Micura Ronald R Tollinger Martin M Kreutz Christoph C
Angewandte Chemie (International ed. in English) 20141117 2
An NMR-based approach to characterizing the binding kinetics of ligand molecules to biomolecules, like RNA or proteins, by ligand-detected Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments is described. A (15)N-modified preQ1 ligand is used to acquire relaxation dispersion experiments in the presence of low amounts of the Fsu class I preQ1 aptamer RNA, and increasing ligand concentrations to probe the RNA small molecule interaction. Our experimental data strongly support the con ...[more]