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Cox-2 inhibition and the composition of inflammatory cell populations during early and mid-time tendon healing.


ABSTRACT: Background:During early tendon healing, the cells within the regenerating tissue are, to a large part, inflammatory leukocytes (CD45+). In a rat Achilles tendon healing model, the inflammation resolves between 5 and 10 days. In the same model, Cox inhibitors (NSAIDs) impair healing when given during the first 5 days, but have a positive effect if given later. We tested the hypothesis that a Cox inhibitor would exert these effects by influencing inflammation, and thereby the composition of the inflammatory cell subpopulations. Methods:Achilles tendon transection was performed in 44 animals. Animals were randomized to either parecoxib or saline injections. Healing was evaluated by mechanical testing day 7 after surgery and by flow cytometry day 3 and 10. Results:Cross-sectional area, peak force and stiffness were reduced by parecoxib 31, 33, and 25% respectively (p=0.005, p=0.002, and p=0.005). By flow cytometry, there was a strong effect of time (p<0.001) on virtually all inflammatory cell subpopulations (CD45, CD11b, CD68, CCR7, CD163, CD206, CD3, CD4), but no significant effect of parecoxib at any time point. Conclusion:The results suggest that the negative effects of Cox inhibitors on tendon healing might be exerted mainly via mechanisms not directly related to inflammatory cells.

SUBMITTER: Blomgran P 

PROVIDER: S-EPMC5725170 | biostudies-literature | 2017 Apr-Jun

REPOSITORIES: biostudies-literature

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Cox-2 inhibition and the composition of inflammatory cell populations during early and mid-time tendon healing.

Blomgran Parmis P   Blomgran Robert R   Ernerudh Jan J   Aspenberg Per P  

Muscles, ligaments and tendons journal 20170401 2


<h4>Background</h4>During early tendon healing, the cells within the regenerating tissue are, to a large part, inflammatory leukocytes (CD45<sup>+</sup>). In a rat Achilles tendon healing model, the inflammation resolves between 5 and 10 days. In the same model, Cox inhibitors (NSAIDs) impair healing when given during the first 5 days, but have a positive effect if given later. We tested the hypothesis that a Cox inhibitor would exert these effects by influencing inflammation, and thereby the co  ...[more]

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