Ontology highlight
ABSTRACT:
SUBMITTER: Monaco S
PROVIDER: S-EPMC5725711 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Monaco Serena S Tailford Louise E LE Juge Nathalie N Angulo Jesus J
Angewandte Chemie (International ed. in English) 20171023 48
Saturation transfer difference (STD) NMR spectroscopy is extensively used to obtain epitope maps of ligands binding to protein receptors, thereby revealing structural details of the interaction, which is key to direct lead optimization efforts in drug discovery. However, it does not give information about the nature of the amino acids surrounding the ligand in the binding pocket. Herein, we report the development of the novel method differential epitope mapping by STD NMR (DEEP-STD NMR) for iden ...[more]