Ontology highlight
ABSTRACT:
SUBMITTER: Weeks AM
PROVIDER: S-EPMC5726896 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Weeks Amy M AM Wells James A JA
Nature chemical biology 20171120 1
Enzyme-catalyzed peptide ligation is a powerful tool for site-specific protein bioconjugation, but stringent enzyme-substrate specificity limits its utility. We developed an approach for comprehensively characterizing peptide ligase specificity for N termini using proteome-derived peptide libraries. We used this strategy to characterize the ligation efficiency for >25,000 enzyme-substrate pairs in the context of the engineered peptide ligase subtiligase and identified a family of 72 mutant subti ...[more]