Ontology highlight
ABSTRACT:
SUBMITTER: Berk C
PROVIDER: S-EPMC7894349 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Berk Christian C Wang Yuluan Y Laski Artur A Tsagkris Stylianos S Hall Jonathan J
FEBS letters 20201116 2
Identifying the targetome of a microRNA is key for understanding its functions. Cross-linking and immunoprecipitation (CLIP) methods capture native miRNA-mRNA interactions in cells. Some of these methods yield small amounts of chimeric miRNA-mRNA sequences via ligation of 5'-phosphorylated RNAs produced during the protocol. Here, we introduce chemically synthesized microRNAs (miRNAs) bearing 2'-, 3'-cyclic phosphate groups, as part of a new CLIP method that does not require 5'-phosphorylation fo ...[more]