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Polymorphic design of DNA origami structures through mechanical control of modular components.

ABSTRACT: Scaffolded DNA origami enables the bottom-up fabrication of diverse DNA nanostructures by designing hundreds of staple strands, comprised of complementary sequences to the specific binding locations of a scaffold strand. Despite its exceptionally high design flexibility, poor reusability of staples has been one of the major hurdles to fabricate assorted DNA constructs in an effective way. Here we provide a rational module-based design approach to create distinct bent shapes with controllable geometries and flexibilities from a single, reference set of staples. By revising the staple connectivity within the desired module, we can control the location, stiffness, and included angle of hinges precisely, enabling the construction of dozens of single- or multiple-hinge structures with the replacement of staple strands up to 12.8% only. Our design approach, combined with computational shape prediction and analysis, can provide a versatile and cost-effective procedure in the design of DNA origami shapes with stiffness-tunable units.

SUBMITTER: Lee C

PROVIDER: S-EPMC5727162 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Polymorphic design of DNA origami structures through mechanical control of modular components.

Lee Chanseok C Lee Jae Young JY Kim Do-Nyun DN

Nature communications 20171212 1

Scaffolded DNA origami enables the bottom-up fabrication of diverse DNA nanostructures by designing hundreds of staple strands, comprised of complementary sequences to the specific binding locations of a scaffold strand. Despite its exceptionally high design flexibility, poor reusability of staples has been one of the major hurdles to fabricate assorted DNA constructs in an effective way. Here we provide a rational module-based design approach to create distinct bent shapes with controllable geo ...[more]

PMID: 29233997

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Dataset Information

Polymorphic design of DNA origami structures through mechanical control of modular components.

Publications

Polymorphic design of DNA origami structures through mechanical control of modular components.

Similar Datasets

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