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Cell shape information is transduced through tension-independent mechanisms.

ABSTRACT: The shape of a cell within tissues can represent the history of chemical and physical signals that it encounters, but can information from cell shape regulate cellular phenotype independently? Using optimal control theory to constrain reaction-diffusion schemes that are dependent on different surface-to-volume relationships, we find that information from cell shape can be resolved from mechanical signals. We used microfabricated 3-D biomimetic chips to validate predictions that shape-sensing occurs in a tension-independent manner through integrin ?3 signaling pathway in human kidney podocytes and smooth muscle cells. Differential proteomics and functional ablation assays indicate that integrin ?3 is critical in transduction of shape signals through ezrin-radixin-moesin (ERM) family. We used experimentally determined diffusion coefficients and experimentally validated simulations to show that shape sensing is an emergent cellular property enabled by multiple molecular characteristics of integrin ?3. We conclude that 3-D cell shape information, transduced through tension-independent mechanisms, can regulate phenotype.

SUBMITTER: Ron A

PROVIDER: S-EPMC5732205 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Cell shape information is transduced through tension-independent mechanisms.

Ron Amit A Azeloglu Evren U EU Calizo Rhodora C RC Hu Mufeng M Bhattacharya Smiti S Chen Yibang Y Jayaraman Gomathi G Lee Sunwoo S Neves-Zaph Susana R SR Li Hong H Gordon Ronald E RE He John C JC Hone James C JC Iyengar Ravi R

Nature communications 20171215 1

The shape of a cell within tissues can represent the history of chemical and physical signals that it encounters, but can information from cell shape regulate cellular phenotype independently? Using optimal control theory to constrain reaction-diffusion schemes that are dependent on different surface-to-volume relationships, we find that information from cell shape can be resolved from mechanical signals. We used microfabricated 3-D biomimetic chips to validate predictions that shape-sensing occ ...[more]

PMID: 29247198

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Cell shape information is transduced through tension-independent mechanisms.

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Cell shape information is transduced through tension-independent mechanisms.

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