Construction of Stable Fluorescent Reporter Plasmids for Use in Staphylococcus aureus.
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ABSTRACT:
SUBMITTER: Rodriguez MD
PROVIDER: S-EPMC5735104 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Publications
Construction of Stable Fluorescent Reporter Plasmids for Use in <i>Staphylococcus aureus</i>.
Rodriguez Michelle D MD Paul Zubin Z Wood Charles E CE Rice Kelly C KC Triplett Eric W EW
Frontiers in microbiology 20171214
Here, the genes encoding three different fluorescent proteins were cloned into the stably maintained <i>Staphylococcus aureus</i> shuttle vector pKK30. The resulting plasmids were transformed into two <i>S. aureus</i> strains; SH1000 and RN4220. Stability assays illustrated that the three recombinant plasmids retained near 100% maintenance <i>in vitro</i> for 160 generations. <i>S. aureus</i> strain SH1000 expressing green fluorescent protein was then inoculated in an ovine model and <i>in vivo< ...[more]
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