Ontology highlight
ABSTRACT:
SUBMITTER: Webster PJ
PROVIDER: S-EPMC5736347 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Webster Peter John PJ Littlejohns Anna Tiffany AT Gaunt Hannah Jane HJ Prasad K Raj KR Beech David John DJ Burke Dermot Anthony DA
Cell cycle (Georgetown, Tex.) 20171109 22
AZD1775 is a small molecule WEE1 inhibitor used in combination with DNA-damaging agents to cause premature mitosis and cell death in p53-mutated cancer cells. Here we sought to determine the mechanism of action of AZD1775 in combination with chemotherapeutic agents in light of recent findings that AZD1775 can cause double-stranded DNA (DS-DNA) breaks. AZD1775 significantly improved the cytotoxicity of 5-FU in a p53-mutated colorectal cancer cell line (HT29 cells), decreasing the IC<sub>50</sub> ...[more]