Ontology highlight
ABSTRACT:
SUBMITTER: Shepherd TR
PROVIDER: S-EPMC5737471 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Shepherd Tyson R TR Du Liping L Liljeruhm Josefine J Samudyata Wang Jinfan J Sjödin Marcus O D MOD Wetterhall Magnus M Yomo Tetsuya T Forster Anthony C AC
Nucleic acids research 20171001 18
Two of the many goals of synthetic biology are synthesizing large biochemical systems and simplifying their assembly. While several genes have been assembled together by modular idempotent cloning, it is unclear if such simplified strategies scale to very large constructs for expression and purification of whole pathways. Here we synthesize from oligodeoxyribonucleotides a completely de-novo-designed, 58-kb multigene DNA. This BioBrick plasmid insert encodes 30 of the 31 translation factors of t ...[more]