Ontology highlight
ABSTRACT:
SUBMITTER: Caufield JH
PROVIDER: S-EPMC5737776 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Caufield J H JH Sakhawalkar Neha N Uetz Peter P
Methods (San Diego, Calif.) 20121208 4
Two-hybrid (Y2H) assays are available in a variety of different versions, including bacterial, yeast, and mammalian systems. However, even when done exclusively in yeast, multiple different host strains, vectors, reporter genes, or protocols can be used. Here we systematically compare protein-protein interactions (PPIs) from several previously published Y2H datasets. PPIs of a human gold-standard dataset were generated by Y2H assays as well as other methods such as LUMIER or protein fragment com ...[more]