Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing.
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ABSTRACT:
SUBMITTER: Wang K
PROVIDER: S-EPMC5741047 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Publications
Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing.
Wang Kepin K Jin Qin Q Ruan Degong D Yang Yi Y Liu Qishuai Q Wu Han H Zhou Zhiwei Z Ouyang Zhen Z Liu Zhaoming Z Zhao Yu Y Zhao Bentian B Zhang Quanjun Q Peng Jiangyun J Lai Chengdan C Fan Nana N Liang Yanhui Y Lan Ting T Li Nan N Wang Xiaoshan X Wang Xinlu X Fan Yong Y Doevendans Pieter A PA Sluijter Joost P G JPG Liu Pentao P Li Xiaoping X Lai Liangxue L
Genome research 20171116 12
Despite being time-consuming and costly, generating genome-edited pigs holds great promise for agricultural, biomedical, and pharmaceutical applications. To further facilitate genome editing in pigs, we report here establishment of a pig line with Cre-inducible Cas9 expression that allows a variety of ex vivo genome editing in fibroblast cells including single- and multigene modifications, chromosome rearrangements, and efficient in vivo genetic modifications. As a proof of principle, we were ab ...[more]
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