Ontology highlight
ABSTRACT:
SUBMITTER: Rouck JE
PROVIDER: S-EPMC5741078 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Rouck John Edward JE Biggs Bradley Walters BW Kambalyal Amogh A Arnold William R WR De Mey Marjan M Ajikumar Parayil Kumaran PK Das Aditi A
Protein expression and purification 20170118
Taxadiene-5α-Hydroxylase (CYP725A4) is a membrane-bound plant cytochrome P450 that catalyzes the oxidation of taxadiene to taxadiene-5α-ol. This oxidation is a key step in the production of the valuable cancer therapeutic and natural plant product, taxol. In this work, we report the bacterial expression and purification of six different constructs of CYP725A4. All six of these constructs are N-terminally modified and three of them are fused to cytochrome P450 reductase to form a chimera construc ...[more]