Ontology highlight
ABSTRACT:
SUBMITTER: Pinilla-Macua I
PROVIDER: S-EPMC5741375 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
eLife 20171221
Despite a well-established role for the epidermal growth factor receptor (EGFR) in tumorigenesis, EGFR activities and endocytosis in tumors in vivo have not been studied. We labeled endogenous EGFR with GFP by genome-editing of human oral squamous cell carcinoma cells, which were used to examine EGFR-GFP behavior in mouse tumor xenografts in vivo. Intravital multiphoton imaging, confocal imaging of cryosections and biochemical analysis revealed that localization and trafficking patterns, as well ...[more]