Ontology highlight
ABSTRACT:
SUBMITTER: Martineau M
PROVIDER: S-EPMC5741633 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Martineau Magalie M Guzman Raul E RE Fahlke Christoph C Klingauf Jürgen J
Nature communications 20171222 1
Glutamate is the major excitatory transmitter in the vertebrate nervous system. To maintain synaptic efficacy, recycling synaptic vesicles (SV) are refilled with glutamate by vesicular glutamate transporters (VGLUTs). The dynamics and mechanism of glutamate uptake in intact neurons are still largely unknown. Here, we show by live-cell imaging with pH- and chloride-sensitive fluorescent probes in cultured hippocampal neurons of wild-type and VGLUT1-deficient mice that in SVs VGLUT functions as a ...[more]