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Stimulated Raman scattering spectroscopic optical coherence tomography.

ABSTRACT: We integrate spectroscopic optical coherence tomography (SOCT) with stimulated Raman scattering (SRS) to enable simultaneously multiplexed spatial and spectral imaging with sensitivity to many endogenous biochemical species that play an important role in biology and medicine. The combined approach, termed SRS-SOCT, overcomes the limitations of each individual method. Ultimately, SRS-SOCT has the potential to achieve fast, volumetric, and highly sensitive label-free molecular imaging. We demonstrate the approach by imaging excised human adipose tissue and detecting the lipids' Raman signatures in the high-wavenumber region.

SUBMITTER: Robles FE

PROVIDER: S-EPMC5749255 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Stimulated Raman scattering spectroscopic optical coherence tomography.

Robles Francisco E FE Zhou Kevin C KC Fischer Martin C MC Warren Warren S WS

Optica 20170214 2

We integrate spectroscopic optical coherence tomography (SOCT) with stimulated Raman scattering (SRS) to enable simultaneously multiplexed spatial <i>and</i> spectral imaging with sensitivity to many endogenous biochemical species that play an important role in biology and medicine. The combined approach, termed SRS-SOCT, overcomes the limitations of each individual method. Ultimately, SRS-SOCT has the potential to achieve fast, volumetric, and highly sensitive label-free molecular imaging. We d ...[more]

PMID: 29302608

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