Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position.

ABSTRACT: Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon University of California base formation during protein synthesis. Our results suggest that codon:anticodon base pairing is subject to tighter constraints at the middle position than at the 5'- and 3'-positions, and further suggest a sequential mechanism of formation of the three base pairs in the codon:anticodon helix.

SUBMITTER: Liu W 

PROVIDER: S-EPMC5753802 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature