Ontology highlight
ABSTRACT:
SUBMITTER: Martin-Marcos P
PROVIDER: S-EPMC5756025 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Martin-Marcos Pilar P Zhou Fujun F Karunasiri Charm C Zhang Fan F Dong Jinsheng J Nanda Jagpreet J Kulkarni Shardul D SD Sen Neelam Dabas ND Tamame Mercedes M Zeschnigk Michael M Lorsch Jon R JR Hinnebusch Alan G AG
eLife 20171205
The translation pre-initiation complex (PIC) scans the mRNA for an AUG codon in favorable context, and AUG recognition stabilizes a closed PIC conformation. The unstructured N-terminal tail (NTT) of yeast eIF1A deploys five basic residues to contact tRNA<sub>i</sub>, mRNA, or 18S rRNA exclusively in the closed state. Interestingly, <i>EIF1AX</i> mutations altering the human eIF1A NTT are associated with uveal melanoma (UM). We found that substituting all five basic residues, and seven UM-associa ...[more]