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Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181.

ABSTRACT: PURPOSE:FCGRT encodes the alpha-chain component of the neonatal Fc receptor (FcRn). FcRn is critical for the trafficking of endogenous and exogenous IgG molecules and albumin in various tissues. Few regulators of FcRn expression have been identified. We investigated the epigenetic regulation of FcRn by two microRNAs (hsa-miR-3181 and hsa-miR-3136-3p) acting on FCGRT. METHODS:The binding of candidate microRNAs to the 3'-untranslated region of FCGRT was evaluated using luciferase reporter constructs in CHO cells. The effect of microRNAs on FCGRT mRNA and FcRn protein expression was evaluated using specific microRNA mimics and inhibitor transfections in A549, HEK293 and HepG2 cells. RESULTS:Hsa-miR-3181 mimic reduced luciferase reporter activity by 70.1% (10 nM, P

SUBMITTER: Ferguson DC

PROVIDER: S-EPMC5757240 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181.

Ferguson Daniel C DC Blanco Javier G JG

Pharmaceutical research 20180104 1

<h4>Purpose</h4>FCGRT encodes the alpha-chain component of the neonatal Fc receptor (FcRn). FcRn is critical for the trafficking of endogenous and exogenous IgG molecules and albumin in various tissues. Few regulators of FcRn expression have been identified. We investigated the epigenetic regulation of FcRn by two microRNAs (hsa-miR-3181 and hsa-miR-3136-3p) acting on FCGRT.<h4>Methods</h4>The binding of candidate microRNAs to the 3'-untranslated region of FCGRT was evaluated using luciferase re ...[more]

PMID: 29302759

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