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Bacterial cyclic ?-(1,2)-glucans sequester iron to protect against iron-induced toxicity.


ABSTRACT: Cellular iron homeostasis is critical for survival and growth. Bacteria employ a variety of strategies to sequester iron from the environment and to store intracellular iron surplus that can be utilized in iron-restricted conditions while also limiting the potential for the production of iron-induced reactive oxygen species (ROS). Here, we report that membrane-derived oligosaccharide (mdo) glucan, an intrinsic component of Gram-negative bacteria, sequesters the ferrous form of iron. Iron-binding, uptake, and localization experiments indicated that both secreted and periplasmic ?-(1,2)-glucans bind iron specifically and promote growth under iron-restricted conditions. Xanthomonas campestris and Escherichia coli mutants blocked in the production of ?-(1,2)-glucan accumulate low amounts of intracellular iron under iron-restricted conditions, whereas they exhibit elevated ROS production and sensitivity under iron-replete conditions. Our results reveal a critical role of glucan in intracellular iron homeostasis conserved in Gram-negative bacteria.

SUBMITTER: Javvadi S 

PROVIDER: S-EPMC5757255 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Bacterial cyclic β-(1,2)-glucans sequester iron to protect against iron-induced toxicity.

Javvadi Sreegowrinadh S   Pandey Sheo Shankar SS   Mishra Amita A   Pradhan Binod Bihari BB   Chatterjee Subhadeep S  

EMBO reports 20171208 1


Cellular iron homeostasis is critical for survival and growth. Bacteria employ a variety of strategies to sequester iron from the environment and to store intracellular iron surplus that can be utilized in iron-restricted conditions while also limiting the potential for the production of iron-induced reactive oxygen species (ROS). Here, we report that membrane-derived oligosaccharide (mdo) glucan, an intrinsic component of Gram-negative bacteria, sequesters the ferrous form of iron. Iron-binding  ...[more]

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