Ontology highlight
ABSTRACT:
SUBMITTER: Canny MD
PROVIDER: S-EPMC5762392 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Nature biotechnology 20171127 1
Programmable nucleases, such as Cas9, are used for precise genome editing by homology-dependent repair (HDR). However, HDR efficiency is constrained by competition from other double-strand break (DSB) repair pathways, including non-homologous end-joining (NHEJ). We report the discovery of a genetically encoded inhibitor of 53BP1 that increases the efficiency of HDR-dependent genome editing in human and mouse cells. 53BP1 is a key regulator of DSB repair pathway choice in eukaryotic cells and fun ...[more]