Ontology highlight
ABSTRACT:
SUBMITTER: DiNapoli SE
PROVIDER: S-EPMC7144937 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
DiNapoli Sara E SE Martinez-McFaline Raul R Gribbin Caitlin K CK Wrighton Paul J PJ Balgobin Courtney A CA Nelson Isabel I Leonard Abigail A Maskin Carolyn R CR Shwartz Arkadi A Quenzer Eleanor D ED Mailhiot Darya D Kao Clara C McConnell Sean C SC de Jong Jill L O JLO Goessling Wolfram W Houvras Yariv Y
Nucleic acids research 20200401 7
CRISPR/Cas9 has become a powerful tool for genome editing in zebrafish that permits the rapid generation of loss of function mutations and the knock-in of specific alleles using DNA templates and homology directed repair (HDR). We examined the efficiency of synthetic, chemically modified gRNAs and demonstrate induction of indels and large genomic deletions in combination with recombinant Cas9 protein. We developed an in vivo genetic assay to measure HDR efficiency and we utilized this assay to t ...[more]