Ontology highlight
ABSTRACT:
SUBMITTER: Rovira AR
PROVIDER: S-EPMC5766281 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Rovira Alexander R AR Fin Andrea A Tor Yitzhak Y
Journal of the American Chemical Society 20171027 44
The synthesis, photophysics, and biochemical utility of a fluorescent NAD<sup>+</sup> analogue based on an isothiazolo[4,3-d]pyrimidine core (N<sup>tz</sup>AD<sup>+</sup>) are described. Enzymatic reactions, photophysically monitored in real time, show N<sup>tz</sup>AD<sup>+</sup> and N<sup>tz</sup>ADH to be substrates for yeast alcohol dehydrogenase and lactate dehydrogenase, respectively, with reaction rates comparable to that of the native cofactors. A drop in fluorescence is seen as N<sup>tz ...[more]