Ontology highlight
ABSTRACT:
SUBMITTER: Li S
PROVIDER: S-EPMC5767123 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Li Sujun S Dabir Aditi A Misal Santosh A SA Tang Haixu H Radivojac Predrag P Reilly James P JP
Journal of proteome research 20160927 10
Peptide amidination labeling using S-methyl thioacetimidate (SMTA) is investigated in an attempt to increase the number and types of peptides that can be detected in a bottom-up proteomics experiment. This derivatization method affects the basicity of lysine residues and is shown here to significantly impact the idiosyncracies of peptide fragmentation and peptide detectability. The unique and highly reproducible fragmentation properties of SMTA-labeled peptides, such as the strong propensity for ...[more]