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Accurate detection of chemical modifications in RNA by mutational profiling (MaP) with ShapeMapper 2.

ABSTRACT: Mutational profiling (MaP) enables detection of sites of chemical modification in RNA as sequence changes during reverse transcription (RT), subsequently read out by massively parallel sequencing. We introduce ShapeMapper 2, which integrates careful handling of all classes of adduct-induced sequence changes, sequence variant correction, basecall quality filters, and quality-control warnings to now identify RNA adduct sites as accurately as achieved by careful manual analysis of electrophoresis data, the prior highest-accuracy standard. MaP and ShapeMapper 2 provide a robust, experimentally concise, and accurate approach for reading out nucleic acid chemical probing experiments.

SUBMITTER: Busan S

PROVIDER: S-EPMC5769742 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Accurate detection of chemical modifications in RNA by mutational profiling (MaP) with ShapeMapper 2.

Busan Steven S Weeks Kevin M KM

RNA (New York, N.Y.) 20171107 2

Mutational profiling (MaP) enables detection of sites of chemical modification in RNA as sequence changes during reverse transcription (RT), subsequently read out by massively parallel sequencing. We introduce ShapeMapper 2, which integrates careful handling of all classes of adduct-induced sequence changes, sequence variant correction, basecall quality filters, and quality-control warnings to now identify RNA adduct sites as accurately as achieved by careful manual analysis of electrophoresis d ...[more]

PMID: 29114018

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