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Random-access scanning microscopy for 3D imaging in awake behaving animals.

ABSTRACT: Understanding how neural circuits process information requires rapid measurements of activity from identified neurons distributed in 3D space. Here we describe an acousto-optic lens two-photon microscope that performs high-speed focusing and line scanning within a volume spanning hundreds of micrometers. We demonstrate its random-access functionality by selectively imaging cerebellar interneurons sparsely distributed in 3D space and by simultaneously recording from the soma, proximal and distal dendrites of neocortical pyramidal cells in awake behaving mice.

SUBMITTER: Nadella KM

PROVIDER: S-EPMC5769813 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Random-access scanning microscopy for 3D imaging in awake behaving animals.

Nadella K M Naga Srinivas KM Roš Hana H Baragli Chiara C Griffiths Victoria A VA Konstantinou George G Koimtzis Theo T Evans Geoffrey J GJ Kirkby Paul A PA Silver R Angus RA

Nature methods 20161017 12

Understanding how neural circuits process information requires rapid measurements of activity from identified neurons distributed in 3D space. Here we describe an acousto-optic lens two-photon microscope that performs high-speed focusing and line scanning within a volume spanning hundreds of micrometers. We demonstrate its random-access functionality by selectively imaging cerebellar interneurons sparsely distributed in 3D space and by simultaneously recording from the soma, proximal and distal ...[more]

PMID: 27749836

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