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Random access parallel microscopy.

ABSTRACT: We introduce a random-access parallel (RAP) imaging modality that uses a novel design inspired by a Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous image capture of multiple petri dishes and random-access imaging with sub-millisecond switching times at the full resolution of the camera. This enables the RAP system to capture long-duration records from different samples in parallel, which is not possible using conventional automated microscopes. The system is demonstrated by continuously imaging multiple cardiac monolayer and Caenorhabditis elegans preparations.

SUBMITTER: Ashraf M

PROVIDER: S-EPMC7843131 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Random access parallel microscopy.

Ashraf Mishal M Mohanan Sharika S Sim Byu Ri BR Tam Anthony A Rahemipour Kiamehr K Brousseau Denis D Thibault Simon S Corbett Alexander D AD Bub Gil G

eLife 20210112

We introduce a random-access parallel (RAP) imaging modality that uses a novel design inspired by a Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous image capture of multiple petri dishes and random-access imaging with sub-millisecond switching times at the full resolution of the camera. This enables the RAP system to capture long-duration records from different samples in parallel, which is not possible usi ...[more]

PMID: 33432922

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