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A Chemoenzymatic Histology Method for O-GlcNAc Detection.

ABSTRACT: Modification of nuclear and cytoplasmic proteins by the addition or removal of O-GlcNAc dynamically impacts multiple biological processes. Here, we present the development of a chemoenzymatic histology method for the detection of O-GlcNAc in tissue specimens. We applied this method to screen murine organs, uncovering specific O-GlcNAc distribution patterns in different tissue structures. We then utilized our histology method for O-GlcNAc detection in human brain specimens from healthy donors and donors with Alzheimer's disease and found higher levels of O-GlcNAc in specimens from healthy donors. We also performed an analysis using a multiple cancer tissue array, uncovering different O-GlcNAc levels between healthy and cancerous tissues, as well as different O-GlcNAc cellular distributions within certain tissue specimens. This chemoenzymatic histology method therefore holds great potential for revealing the biology of O-GlcNAc in physiopathological processes.

SUBMITTER: Aguilar AL

PROVIDER: S-EPMC5771404 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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A Chemoenzymatic Histology Method for O-GlcNAc Detection.

Aguilar Aime Lopez AL Hou Xiaomeng X Wen Liuqing L Wang Peng G PG Wu Peng P

Chembiochem : a European journal of chemical biology 20171205 24

Modification of nuclear and cytoplasmic proteins by the addition or removal of O-GlcNAc dynamically impacts multiple biological processes. Here, we present the development of a chemoenzymatic histology method for the detection of O-GlcNAc in tissue specimens. We applied this method to screen murine organs, uncovering specific O-GlcNAc distribution patterns in different tissue structures. We then utilized our histology method for O-GlcNAc detection in human brain specimens from healthy donors and ...[more]

PMID: 29044951

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