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Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins.


ABSTRACT: Glycan-binding proteins (GBPs) play critical roles in diverse cellular functions such as cell adhesion, signal transduction and immune response. Studies of the interaction between GBPs and glycans have been hampered by the availability of high throughput and high-content technologies. Here we report multiplex glycan bead array (MGBA) that allows simultaneous analyses of 384 samples and up to 500 glycans in a single assay. The specificity, sensitivity and reproducibility of MGBA are evaluated using 39 plant lectins, 13 recombinant anti-glycan antibodies, and mammalian GBPs. We demonstrate the utility of this platform by the analyses of natural anti-glycan IgM and IgG antibodies in 961 human serum samples and the discovery of anti-glycan antibody biomarkers for ovarian cancer. Our data indicate that the MGBA platform is particularly suited for large population-based studies that require the analyses of large numbers of samples and glycans.

SUBMITTER: Purohit S 

PROVIDER: S-EPMC5772357 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins.

Purohit Sharad S   Li Tiehai T   Guan Wanyi W   Song Xuezheng X   Song Jing J   Tian Yanna Y   Li Lei L   Sharma Ashok A   Dun Boying B   Mysona David D   Ghamande Sharad S   Rungruang Bunja B   Cummings Richard D RD   Wang Peng George PG   She Jin-Xiong JX  

Nature communications 20180117 1


Glycan-binding proteins (GBPs) play critical roles in diverse cellular functions such as cell adhesion, signal transduction and immune response. Studies of the interaction between GBPs and glycans have been hampered by the availability of high throughput and high-content technologies. Here we report multiplex glycan bead array (MGBA) that allows simultaneous analyses of 384 samples and up to 500 glycans in a single assay. The specificity, sensitivity and reproducibility of MGBA are evaluated usi  ...[more]

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