Ontology highlight
ABSTRACT:
SUBMITTER: Itzhak DN
PROVIDER: S-EPMC5775508 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Cell reports 20170901 11
We previously developed a mass spectrometry-based method, dynamic organellar maps, for the determination of protein subcellular localization and identification of translocation events in comparative experiments. The use of metabolic labeling for quantification (stable isotope labeling by amino acids in cell culture [SILAC]) renders the method best suited to cells grown in culture. Here, we have adapted the workflow to both label-free quantification (LFQ) and chemical labeling/multiplexing strate ...[more]