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The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio).


ABSTRACT: Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.

SUBMITTER: Usui T 

PROVIDER: S-EPMC5775754 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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The French press: a repeatable and high-throughput approach to exercising zebrafish (<i>Danio rerio</i>).

Usui Takuji T   Noble Daniel W A DWA   O'Dea Rose E RE   Fangmeier Melissa L ML   Lagisz Malgorzata M   Hesselson Daniel D   Nakagawa Shinichi S  

PeerJ 20180117


Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim pe  ...[more]

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