PH-sensitive amide proton transfer effect dominates the magnetization transfer asymmetry contrast during acute ischemia-quantification of multipool contribution to in vivo CEST MRI.

ABSTRACT:

Purpose

To determine the origins of in vivo magnetization transfer asymmetry contrast during acute ischemic stroke, particularly in the diffusion lesion, perfusion lesion, and their mismatch using a middle cerebral artery occlusion rat model of acute stroke.

Methods

Adult male Wistar rats underwent multiparametric MRI of diffusion, perfusion, T₁ , and amide proton transfer (APT) imaging at 4.7 T following a middle cerebral artery occlusion procedure. A multipool Lorentzian model, including the nuclear Overhauser effect, magnetization transfer, direct water saturation, amine and amide chemical exchange saturation transfer effects, was applied for Z-spectrum fitting to determine the sources of in vivo magnetization transfer asymmetry following acute stroke.

Resul

SUBMITTER: Wu Y 

PROVIDER: S-EPMC5775916 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature