Ontology highlight
ABSTRACT:
SUBMITTER: Ryan DE
PROVIDER: S-EPMC5778453 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Ryan Daniel E DE Taussig David D Steinfeld Israel I Phadnis Smruti M SM Lunstad Benjamin D BD Singh Madhurima M Vuong Xuan X Okochi Kenji D KD McCaffrey Ryan R Olesiak Magdalena M Roy Subhadeep S Yung Chong Wing CW Curry Bo B Sampson Jeffrey R JR Bruhn Laurakay L Dellinger Douglas J DJ
Nucleic acids research 20180101 2
CRISPR systems have emerged as transformative tools for altering genomes in living cells with unprecedented ease, inspiring keen interest in increasing their specificity for perfectly matched targets. We have developed a novel approach for improving specificity by incorporating chemical modifications in guide RNAs (gRNAs) at specific sites in their DNA recognition sequence ('guide sequence') and systematically evaluating their on-target and off-target activities in biochemical DNA cleavage assay ...[more]