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Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells.

ABSTRACT: CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency in human primary T cells and CD34(+) hematopoietic stem and progenitor cells. Co-delivering chemically modified sgRNAs with Cas9 mRNA or protein is an efficient RNA- or ribonucleoprotein (RNP)-based delivery method for the CRISPR-Cas system, without the toxicity associated with DNA delivery. This approach is a simple and effective way to streamline the development of genome editing with the potential to accelerate a wide array of biotechnological and therapeutic applications of the CRISPR-Cas technology.

SUBMITTER: Hendel A

PROVIDER: S-EPMC4729442 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells.

Hendel Ayal A Bak Rasmus O RO Clark Joseph T JT Kennedy Andrew B AB Ryan Daniel E DE Roy Subhadeep S Steinfeld Israel I Lunstad Benjamin D BD Kaiser Robert J RJ Wilkens Alec B AB Bacchetta Rosa R Tsalenko Anya A Dellinger Douglas D Bruhn Laurakay L Porteus Matthew H MH

Nature biotechnology 20150629 9

CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency in human primary T cells and CD34(+) hematopoietic stem and progenitor cells. Co-delivering chemically modified sgRNAs with Cas9 mRNA or protein is an efficient RNA- or ribonucleoprotein (RNP)-based delivery method for the CRISPR-Cas system, without t ...[more]

PMID: 26121415

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