Modulating Cell-Surface Receptor Signaling and Ion Channel Functions by In?Situ Glycan Editing.

ABSTRACT: Glycans anchored on cell-surface receptors are active modulators of receptor signaling. A strategy is presented that enforces transient changes to cell-surface glycosylation patterns to tune receptor signaling. This approach, termed in?situ glycan editing, exploits recombinant glycosyltransferases to incorporate monosaccharides with linkage specificity onto receptors in?situ. ?2,3-linked sialic acid or ?1,3-linked fucose added in?situ suppresses signaling through epidermal growth factor receptor and fibroblast growth factor receptor. We also applied the same strategy to regulate the electrical signaling of a potassium ion channel-human ether-à-go-go-related gene channel. Compared to gene editing, no long-term perturbations are introduced to the treated cells. In?situ glycan editing therefore offers a promising approach for studying the dynamic role of specific glycans in membrane receptor signaling and ion channel functions.

SUBMITTER: Jiang H 

PROVIDER: S-EPMC5779621 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature