A Discontinuous Galerkin Model for Fluorescence Loss in Photobleaching.
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ABSTRACT:
SUBMITTER: Hansen CV
PROVIDER: S-EPMC5780497 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
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A Discontinuous Galerkin Model for Fluorescence Loss in Photobleaching.
Hansen Christian V CV Schroll Hans J HJ Wüstner Daniel D
Scientific reports 20180123 1
Fluorescence loss in photobleaching (FLIP) is a modern microscopy method for visualization of transport processes in living cells. This paper presents the simulation of FLIP sequences based on a calibrated reaction-diffusion system defined on segmented cell images. By the use of a discontinuous Galerkin method, the computational complexity is drastically reduced compared to continuous Galerkin methods. Using this approach on green fluorescent protein (GFP), we can determine its intracellular dif ...[more]
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