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Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry.

ABSTRACT: Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its 'melting point'. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based heating device implemented into a 96-well plate reader that is connected to a standard fluorescence spectrometer. We demonstrate its potential to determine the 'melting point' of soluble and membranous proteins at various buffer conditions.

SUBMITTER: Hoeser J

PROVIDER: S-EPMC5780519 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry.

Hoeser Jo J Gnandt Emmanuel E Friedrich Thorsten T

Scientific reports 20180123 1

Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its 'melting point'. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based heating device implemented into a 96-well plate reader that is connected to a standard fluorescence spectrometer. We demonstrate its potential to determine the 'melting point' of sol ...[more]

PMID: 29362420

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